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	Provedor de dados BABT (1) BJID (1) Autor Kritski,Afrânio (2) Becker,Daniela (1) Cortez-Herrera,Elizabeth (1) Fujiwara,Paula I. (1) Huf,Gisele (1) Netto,Antonio Ruffino (1) Oliveira,Martha Maria (1) Rossetti,Maria Lucia Rosa (1) Sperhacke,Rosa Dea (1) Squire,S. Bertel (1) Vieira,Maria Armanda (1) Zaha,Arnaldo (1) Mais... Palavra-chave Tuberculosis (2) Diagnostics (1) Evidence (1) Impact (1) Innovation (1) Internal control (1) Nucleic acid amplification (1) Policy (1) Polymerase Chain Reaction (1) Regulatory agency (1) Uracyl DNA glycosilase (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2013 (1) 2008 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Assessing new strategies for TB diagnosis in low- and middle-income countries BJID Kritski,Afrânio; Fujiwara,Paula I.; Vieira,Maria Armanda; Netto,Antonio Ruffino; Oliveira,Martha Maria; Huf,Gisele; Squire,S. Bertel. In this manuscript, we report the current situation of tuberculosis globally and in Brazil, the need for new strategies toward tuberculosis control, focusing on new diagnostic technologies. Critical comments are given on the state of the art regarding the evaluation of new health technologies, degree of scientific evidence needed, evaluation of clinical impact, cost-effectiveness of incorporation into the health system and the social impact. Tipo: Info:eu-repo/semantics/article Palavras-chave: Tuberculosis; Diagnostics; Impact; Evidence; Policy; Innovation; Regulatory agency. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200015 Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvement of the diagnosis BABT Cortez-Herrera,Elizabeth; Sperhacke,Rosa Dea; Becker,Daniela; Kritski,Afrânio; Zaha,Arnaldo; Rossetti,Maria Lucia Rosa. The aim of this work was to construct and test a plasmidial Internal Control (IC) to detect the inhibition in the PCR test for M. tuberculosis and also its contribution for a Public Health Laboratory routine. The IC was a 600-bp of DNA linked to a plasmid with the same primer sites, allowing the amplification with the 245-bp diagnostic fragment. The amplification of the positive samples rendered the IC and the diagnostic fragment; instead negative samples only showed the IC. A total of 149 tuberculosis samples were studied and introduced the IC to monitor. Results showed 3.3% of the samples without amplification of the IC, suggesting the inhibition. These samples showed results in accordance with the clinical results. The objective of the IC was to... Tipo: Info:eu-repo/semantics/article Palavras-chave: Polymerase Chain Reaction; Tuberculosis; Internal control; Nucleic acid amplification; Uracyl DNA glycosilase. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400006 Registros recuperados: 2 Primeira ... 1 ... Última

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